Investigation of blaIMP-1, blaVIM-1, blaOXA-48 and blaNDM-1 carbapenemase encoding genes among MBL-producing Pseudomonas aeruginosa

Pseudomonas aeruginosa (P. aeruginosa) places among major opportunistic nosocomial pathogen which has developed extensive drug resistance. Due to uncontrolled consumption of antibiotics, multidrug-resistant (MDR) P. species are increasingly isolated from various settings hospitals globally. This research aimed determine the genes reproducing blaOXA-48, blaIMP-1, blaVIM-1 and blaNDM-1 metallo beta lactamase (MBL) MDR isolates. Herein, isolates 200 have been obtained microbiology laboratory burn ward. The antibiotic susceptibility profile was using Disc Diffusion Method (DDM in compliance with CLSI (Clinical Laboratory Standards Institute) advice. Study MBL-bearing strains existence encoding specified by means polymerase chain reaction. In addition, pulsed field gel electrophoresis (PFGE) is performed for typing. this study, 124 (62%) were extended spectrum β-lactamase producer 51 (25.5%) MBL producers. Moreover, 148 (74%) MDR-P. aeruginosa. Additionally, 42 (21%), 21 (10%), 10 (5%) 2 (1%) carried genes, respectively. PFGE showed no genetic relationships study observed high rate hospital settings, though not being outbreak, nearly half them carbapenemase enzymes. Therefore, proper control related infections appropriate prescription antibiotics essential.